《Hepatoprotective effects of Ilex paraguariensis St. Hilaire (Yerba mate)》
《马黛茶提取物对于肝脏保护的科学研究》
作者：Seung-Hee Jang, Md. Akil Hossain
研究单位：韩国庆北国立大学
（Kyungpook National University）
研究目的：
通过以往的研究发现
马黛茶是一种
抗氧化剂、利尿剂、低胆固醇作用剂。
因此，本研究旨在
确定马黛茶提取物
对四氯化碳诱导的大鼠肝损伤的保护作用
还评估提取物的保肝作用的可能机制
并对提取物中的主要成分进行了鉴定
The liver is one of the major organs in
the body thatpredominantly controls
the metabolism of endogenous
as well as exogenous agents
This vital organ performs a significant
function in the evacuation and
detoxification of drug materials.
Plants are the vital source of natural antioxidants
that might be an opportunistic treatment option
for the effective suppression of liver toxicity.
Moreover, the investigation of suitable herbal drug
that might be capable to
replace the chemical one isthe crucial exploration
肝脏是体内主要器官之一
是身体内以代谢功能为主的一个器官
这一重要器官在身体内
排毒、消化方面发挥着重要作用
植物是天然抗氧化剂的重要来源
可能是有效抑制肝脏毒性的
一种天然材料
研究马黛茶是否能够以天然成分
参与到抑制肝脏毒性到其中
是目前马黛茶的一个重要研究方向
①研究材料
The dried leaf (1.5 mm cut)
of Ilex paraguariensis
One part of dried Ilex
paraguariensis was suspended in 10 parts of 70 %
ethanol, and boiled at 85 °C for 5 h.
The supernatant
was collected by passing through
a filter paper. The
extract was concentrated in a rotary evaporator
(BüchiRotavapor R-114, BÜCHI
Labortechnik AG,
Flawil, Switzerland) at 80 °C.
The concentrated
extract was then solidified
in a vacuum freeze drier
(BioTron, Gangneung, Korea)
and stored at -20 °C for
further experiments.
选取巴拉圭冬青的干燥叶
并将干燥的马黛茶悬浮于10份70%乙醇中
在85°C下煮沸5h
通过滤纸收集上清液
然后将浓缩提取物在真空冷冻干燥器中固化
并于-20°C下储存以供进一步实验使用。
②研究对象
Six weeks old 50 male Sprague-Dawley rats
(135 ± 15 g) were obtained from Orient BioInc.
(Gyeonggi-do, Korea).
The animals were acclimatized
in a controlled room at 23 ± 3 °C temperature
and50 ± 10 % of relative humidity
with 12 h light/dark